Discussions

Since the completion of the human genome project, sequencing technology has evolved from labor-intensive and time-consuming capillary-based sequencing to massively parallel next generation sequencing (NGS), which leads to a decreased cost per megabase and an increase in the number and diversity of sequenced genomes.

Human platelet lysate (HPL) is a multifactorial supplement obtained from human platelets, which contains abundant growth factors and cytokines necessary for cell growth and proliferation. In a wide range of applications, HPL is considered a good substitute for animal serum. The replacement of fetal bovine serum (FBS) with HPL can reduce the immunogenicity and remove the transmission of zoonotic diseases, which is beneficial to the clinical application of cell proliferation or production.

Gas exchange analysis of photosynthesis in vivo has become an important tool to measure plant performance, and it is also the core of developing higher yield, water-saving and stress-tolerant crops. The increased availability of commercial plant photosynthesis systems has promoted the expansion of the use range of gas exchange, which is helpful to analyze the response of plants to global environmental changes, such as temperature stress, drought, pollution, elevated atmospheric carbon dioxide concentration and biological stress, which affect the sustainability of agriculture and/or natural ecosystems.

The study of the molecular basis of the aging process and the development of anti-aging therapies has become an emerging area of research in recent years. We recognize the importance of this emerging field and has developed a series of animal models for aging research. We utilize a variety of model organisms for the construction of various age-related disease models and aging models.

With the rise of precision medicine and the popularity of massively parallel gene sequencing technologies, brain tumor gene sequencing has become a common approach in brain tumor research and clinical practice. Genomic mapping plays a key role in the diagnosis and grading of brain tumors, and sequencing then allows researchers to trace brain tumors back to individual gene changes to better provide new clues for treatment and research.

Gene methylation, gene mutations, and protein markers are commonly used biomarkers for brain tumor diagnosis. However, the mechanisms of brain tumorigenesis are complex, and a large number of studies have demonstrated the limitations of relying on single omics, as the analysis of single-level data can only provide little cause-and-effect relationships. In contrast, a multi omics data integration strategy spanning genomic, transcriptomic, proteomic, and metabolomic levels of different cellular functions has the potential to provide unparalleled insights into the underlying biology of brain tumors.

Introduction to Antibiotic-Resistant Bacteria
Antibiotic-resistant bacteria have killed more than a million people worldwide in a single year. This indicates that these superbugs have become a major infectious disease. The main superbugs that we have studied and known more about so far include carbapenem-resistant Enterobacteriaceae (CRE), multidrug-resistant Acinetobacter, Neisseria gonorrhoeae, MRSA, and Clostridium difficile. CRE is usually found in our stomach, but some of these bacteria cause bloodstream infections and are resistant to all antibiotics. Acinetobacter baumannii becomes resistant to antibiotics more quickly than other bacteria. Neisseria gonorrhoeae causes the STD gonorrhea, which in the past was easily treated with antibiotics. However, it is now becoming more resistant to antibiotics.

H. pylori belong to the group of Gram-negative bacteria with outer and inner membranes. H. pylori contains more than 60 outer membrane genes. The most studied adhesins belong to the outer MPs. In addition to adhesins, there are also efflux pump proteins and iron-regulated proteins. The inner MPs of H. pylori include Urel, an important protein for H. pylori survival, and some efflux pump proteins. These outer and inner MPs play important roles in the survival, pathogenesis, and drug resistance of H. pylori. For example, nickel import and export MPs are essential for the survival and pathogenesis of H. pylori as they affect the activity of hydrogenase and urease of H. pylori.

As a fast-growing and innovative company, CD Biosynsis has established a powerful synthetic biology platform to integrate engineering principles and a broad range of methodologies from various disciplines, including biology, bioinformatics, chemistry, physics, mathematics, and computer science. We have built a multidisciplinary team dedicated to developing novel synthetic biology tools and incorporating emerging technologies into our platform, giving us the ability to provide custom synthetic biology services and effective strategies to accelerate our customers’ synthetic biology research and development processes.

Synthetic DNA library refers to a collection of DNA fragments stored in host microorganisms by molecular cloning. Each fragment can be stored in a host organism separately. By rapidly and accurately replicating DNA in host organisms and producing detectable quantities, we can have quick access to many different sequences of interest with ease and efficiency. Synthetic DNA libraries enable us to achieve gene synthesis and cloning faster, more intelligent, and more reliable.